24 Urinary Cortisol

The 24-Hour Urinary Cortisol Test is a laboratory investigation used to measure the total amount of free cortisol excreted in urine over a 24-hour period.

Cortisol is a steroid hormone produced by the adrenal glands. It plays an important role in regulating metabolism, blood pressure, stress responses and the body's natural sleep-wake cycle.

This test helps healthcare professionals evaluate cortisol production and investigate possible hormonal disorders, particularly conditions associated with excessive or insufficient cortisol levels.

What Does This Test Check?

• Total free cortisol excretion over 24 hours

• Abnormally high urinary cortisol levels

• Cortisol production and adrenal gland activity

• Possible hormonal imbalances

Sample Requirements

• Sample Type: 24-Hour Urine

• Collection Period: Complete 24-hour urine collection

• Test Category: Laboratory – Hormonal Assessment

Patient Preparation

• Follow the laboratory's dietary and medication instructions.

• Inform your healthcare provider about steroid medications and supplements.

• Do not stop prescribed medications without medical advice.

• Use the laboratory-approved urine collection container.

• Follow the laboratory's storage and transportation instructions.

24-Hour Urine Collection Instructions

1. Day 1 – Morning: Empty your bladder into the toilet after waking up. Do not collect this first urine. Record the exact time.

2. During the Next 24 Hours: Collect all urine passed throughout the day and night in the designated container.

3. Day 2 – Morning: Collect your final urine sample at exactly the same time as the previous day's starting time.

4. Storage: Store the sample according to laboratory instructions.

5. Submission: Deliver the complete collection to the laboratory as instructed.

Relevant Conditions

• Cushing's Syndrome

• Excess Cortisol Production (Hypercortisolism)

• Adrenal Hormonal Disorders

Important Information

Urinary cortisol results should be interpreted by a qualified healthcare professional alongside symptoms, medical history and other relevant hormonal investigations.

Please confirm test availability, collection container requirements and sample handling instructions with the laboratory before booking.