The AFB (Acid-Fast Bacilli) Culture Test is a specialized laboratory investigation used to detect and identify acid-fast bacteria, particularly Mycobacterium tuberculosis, in clinical specimens.
The test involves culturing the submitted sample under suitable laboratory conditions to identify the presence of mycobacteria. It helps healthcare professionals investigate suspected tuberculosis (TB) and certain other mycobacterial infections.
Presence of Acid-Fast Bacilli (AFB)
Growth of Mycobacterium species
Possible Mycobacterial Infections
Laboratory evidence supporting tuberculosis investigation
Sample Types: Sputum, Urine, Body Fluid, Pus or Swab
Sample Quantity: As specified by the laboratory
Test Category: Laboratory – Microbiology
Follow the laboratory's specific sample collection instructions.
Collect the specimen in an appropriate sterile container.
Avoid contamination during sample collection.
Inform your healthcare provider about any ongoing antibiotic or anti-tuberculosis treatment.
Submit the specimen according to laboratory transportation instructions.
Sputum: Collect a deep-cough sputum specimen in a sterile container, not saliva.
Urine: Follow the laboratory's instructions regarding collection timing and quantity.
Body Fluid: Collection must be performed by a qualified healthcare professional.
Pus: Collect the specimen using an appropriate sterile technique.
Swab: Use the laboratory-approved swab and transport medium.
Pulmonary Tuberculosis
Extrapulmonary Tuberculosis
Mycobacterial Infections
Other Acid-Fast Bacilli Infections
AFB culture may take several weeks because mycobacteria can grow slowly. A negative result does not always exclude infection. Results should be interpreted by a qualified healthcare professional alongside clinical findings and other investigations.
Please confirm test availability, accepted specimen types, sample quantity and collection requirements with the laboratory before booking.
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