AFB Staining

The AFB (Acid-Fast Bacilli) Staining Test is a laboratory microscopy examination used to detect acid-fast bacteria in clinical specimens.

This test uses a special staining technique, commonly known as Ziehl-Neelsen staining, to identify acid-fast organisms under a microscope. It is frequently used as an initial investigation for suspected tuberculosis (TB) and other mycobacterial infections.

What Does This Test Check?

• Presence of Acid-Fast Bacilli (AFB)

• Microscopic evidence of mycobacterial infection

• Acid-fast organisms in submitted specimens

• Findings that may support tuberculosis investigation

Sample Requirements

• Sample Types: Sputum, Urine, Body Fluid, Pus or Swab

• Sample Quantity: As specified by the laboratory

• Test Category: Laboratory – Microbiology

Patient Preparation

• Follow the laboratory's specific sample collection instructions.

• Use an appropriate sterile container for the specimen.

• Avoid contamination during collection.

• Inform your healthcare provider about ongoing antibiotic or anti-tuberculosis treatment.

• Submit the specimen according to laboratory transportation instructions.

Specimen Collection Guidelines

• Sputum: Collect a deep-cough sputum specimen, not saliva.

• Urine: Follow the laboratory's instructions regarding collection timing and quantity.

• Body Fluid: Collection should be performed by a qualified healthcare professional.

• Pus: Collect using an appropriate sterile technique.

• Swab: Use the laboratory-approved swab and transport medium.

Relevant Conditions

• Pulmonary Tuberculosis

• Extrapulmonary Tuberculosis

• Mycobacterial Infections

• Other Acid-Fast Bacilli Infections

Important Information

AFB staining provides microscopic evidence of acid-fast organisms but cannot identify the exact bacterial species. A negative staining result does not completely exclude tuberculosis or other mycobacterial infections. Culture or molecular testing may be recommended for further evaluation.

Please confirm test availability, accepted specimen types, sample quantity and collection requirements with the laboratory before booking.