Zn Staning

Product Description

The Ziehl-Neelsen (ZN) Staining Test is a specialised laboratory examination used to detect Acid-Fast Bacilli (AFB) in clinical samples, particularly when investigating suspected tuberculosis (TB).

This staining technique helps identify acid-fast organisms, including Mycobacterium tuberculosis, the bacterium responsible for tuberculosis. It may also help detect other acid-fast organisms, depending on the sample and clinical context.

The test is commonly used as part of the laboratory investigation of suspected pulmonary tuberculosis.

What Does This Test Check?

The test examines a stained sample under a microscope to identify acid-fast bacilli and may help with:

• Detecting AFB in sputum samples.

• Supporting the investigation of suspected pulmonary tuberculosis.

• Identifying acid-fast organisms in appropriate clinical specimens.

• Supporting further investigation of mycobacterial infections.

Sample Requirements

• Sample Type: Sputum (deep respiratory phlegm).

• Sample Quantity: To be confirmed by the laboratory.

• Collection Container: Sterile, wide-mouth, leak-proof sputum container.

• Important: Saliva or ordinary spit is not an acceptable substitute for a proper sputum sample.

Patient Preparation

• Collection Time: Early morning sputum collection is generally preferred.

• Mouth Rinsing: Rinse the mouth with plain water before collection.

• Collection Method: Take a deep breath and cough deeply to collect sputum from the lungs.

• Sample Quality: Avoid collecting saliva or spit.

• Fasting: Fasting is generally not required unless specifically instructed by the laboratory.

Important Information

• A positive AFB smear does not independently confirm tuberculosis or identify the exact mycobacterial species.

• A negative result does not completely exclude tuberculosis.

• Additional investigations, such as GeneXpert MTB/RIF or mycobacterial culture, may be required.

• Results should be interpreted by a qualified healthcare professional.

Reporting Time: To be confirmed by the laboratory.

Please confirm test availability and sample collection requirements with the laboratory before booking.